C-33 A [c-33a]人宫颈癌细胞

C-33 A 细胞株是N. Auersperg从宫颈癌切片中建立的一系列细胞株(参见ATCC CRL-1594和ATCC CRL-1595)中的一株。取样于66岁女性患者， 细胞一开始就表现出亚二倍体核型及上皮细胞形态。 连续传代可以观察到核型不稳定。 存在成视网膜细胞瘤蛋白(pRB)，但大小不正常。 P53表达上调，且有一个273位密码子的点突变导致Arg -> Cys的替换。 人乳头瘤病毒DNA及RNA阴性。

组织来源/Tissue and Cell Type： 宫颈癌

形态/Morphology： 上皮细胞样，贴壁生长；

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%MEM(MD0300)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：MEM完全培养基（货号：MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件   基础培养基+5%DMSO+20%FBS

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用。

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