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产品描述/Products Description
E006AA是一种自发的永生化细胞系，来源于一名非裔美国人后裔体内的Gleason 6局限性前列腺癌。这种细胞系异种移植到完整或阉割的雄性NOG-SCID小鼠体内生长同样良好，且在裸鼠中无致瘤性。E006AA应与E006AA-ht并行用于前列腺癌早期建模。
培养基：前列腺上皮细胞培养基
Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.
培养和冻存方式：
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.
说明：产品仅供科研使用
