SU-DHL-2人B细胞淋巴瘤细胞

此细胞来源于71岁白种女性，这些细胞大多是超二倍体，有51条染色体的尖锐模式数；偶尔也可以看到超四倍体范围内的细胞。在A、B、C和F组中，始终观察到一条微小的标记染色体，染色体数目增加。此细胞非特异性酯酶和酸性磷酸酶阳性。这些细胞对EB病毒核抗原（EBNA-）呈阴性。细胞表面Ig阴性（sIG-）。据报道，这些细胞呈E-玫瑰花结阴性。曾在ATCC通过PCR证实该细胞系存在EB病毒DNA序列。

组织来源：B淋巴细胞

形态：淋巴母细胞样 ，悬浮 多细胞聚集体 生长

培养基配方：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：1640完全培养基（MD0201）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

PubMed=4140017; DOI=10.1002/1097-0142(197412)34:6
Epstein A.L., Kaplan H.S.
Biology of the human malignant lymphomas. I. Establishment in continuous cell culture and heterotransplantation of diffuse histiocytic lymphomas.
Cancer 34:1851-1872(1974)

PubMed=214220; DOI=10.1002/1097-0142(197811)42:5
Epstein A.L., Levy R., Kim H., Henle W., Henle G., Kaplan H.S.
Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines.
Cancer 42:2379-2391(1978)

PubMed=83902; DOI=10.1002/1097-0142(197901)43:1
Kaplan H.S., Goodenow R.S., Gartner S., Bieber M.M.
Biology and virology of the human malignant lymphomas: 1st Milford D Schulz Lecture.
Cancer 43:1-24(1979)

PubMed=371794
Epstein A.L., Kaplan H.S.
Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines.
Cancer Res. 39:1748-1759(1979)

PubMed=3881165; DOI=10.1016/0165-4608(85)90186-4
Hecht B.K., Epstein A.L., Berger C.S., Kaplan H.S., Hecht F.
Histiocytic lymphoma cell lines: immunologic and cytogenetic studies.
Cancer Genet. Cytogenet. 14:205-218(1985)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

PubMed=15356658; DOI=10.1038/sj.leu.2403465
Drexler H.G., MacLeod R.A.F.
Malignant hematopoietic cell lines: in vitro models for the study of anaplastic large-cell lymphoma.
Leukemia 18:1569-1571(2004)

PubMed=21179087; DOI=10.1038/nature09671
Ngo V.N., Young R.M., Schmitz R., Jhavar S., Xiao W.-M., Lim K.-H., Kohlhammer H., Xu W.-H., Yang Y.-D., Zhao H., Shaffer A.L., Romesser P., Wright G., Powell J.I., Rosenwald A., Muller-Hermelink H.-K., Ott G., Gascoyne R.D., Connors J.M., Rimsza L.M., Campo E., Jaffe E.S., Delabie J., Smeland E.B., Fisher R.I., Braziel R.M., Tubbs R.R., Cook J.R., Weisenburger D.D., Chan W.C., Staudt L.M.
Oncogenically active MYD88 mutations in human lymphoma.
Nature 470:115-119(2011)