3T3-L1小鼠胚胎成纤维细胞

L1是通过克隆分离得到的3T3 (swiss小白鼠)的连续亚株。 3T3- l1是一种来源于(小鼠)3T3细胞的细胞系，用于脂肪组织的生物学研究。3T3-L1细胞具有成纤维细胞样的形态，但在适当的条件下，细胞分化成脂肪细胞样的表型.脂肪细胞形态的3T3-L1细胞增加甘油三酯的合成和积累，获得脂肪细胞的印戒状外观。这些细胞也对脂肪和脂类激素以及药物敏感，包括肾上腺素、异丙肾上腺素和胰岛素。 经无支原体鉴定入库。

组织来源 Tissue and Cell Type 胚胎

形态/Morphology 成纤维细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions:

90%DMEM(MD0100)+10%小牛血清(SD0400)+G/A (SD0038)

DMEM完全培养基(MD0101)

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

DOI=10.1016/0092-8674(74)90126-3

Green H., Kehinde O.

Sublines of mouse 3T3 cells that accumulate lipid.

Cell 1:113-116(1974)

PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0

Green H., Meuth M.

An established pre-adipose cell line and its differentiation in culture.

Cell 3:127-133(1974)

PubMed=165899; DOI=10.1016/0092-8674(75)90087-2

Green H., Kehinde O.

An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.

Cell 5:19-27(1975)

Patent=US4003789

Green H.

Triglyceride-accumulating clonal cell line.

Patent number US4003789, 18-Jan-1977

PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6

Levine J.F., Stockdale F.E.

3T3-L1 adipocytes promote the growth of mammary epithelium.

Exp. Cell Res. 151:112-122(1984)

PubMed=28176856; DOI=10.1038/srep42104

Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.

Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.

Sci. Rep. 7:42104-42104(2017)