RWPE-1人正常前列腺上皮细胞

一位54岁白人男性的正常前列腺组织切片的周围区域的上皮细胞用单拷贝的人乳头瘤病毒18（HPV-18）进行转化，建立了RWPE-1。据报道，RWPE-1细胞在三维Matrigel培养时，在雄激素刺激下，形成腺胞（acini）并向培养基中分泌PSA。当与Matrigel或基质细胞混合注射雄性裸鼠时，RWPE-1细胞也能形成腺胞并产生PSA。来源于RWPE-1的细胞用Kirstin鼠肉瘤病毒（Ki-MuSV）转染Ki-ras基因，建立了能成瘤的RWPE-2细胞和 RWPE2-W99细胞。另外，用N-甲醇-N-硝基脲（MNU）处理RWPE-1，建立了一系列模拟前列腺癌进程中不同时期的成瘤细胞株。它们分别是WPE1-NA22, WPE1-NB14, WPE1-NB11和 WPE1-NB26细胞。据报道，RWPE-1细胞经过检测后发现乙肝病毒、丙肝病毒和人免疫缺陷病毒都呈阴性。PCR证实该细胞系HPV病毒DNA序列呈阳性。经无支原体/STR鉴定入库。

组织来源 Tissue and Cell Type  前列腺

形态/Morphology 上皮细胞样，贴壁生长

培养基和添加剂/Complete Growth Medium and Culture Conditions

Keratinocyte-SFM (Invitrogen, 17005-042)

K-SFM（Invitrogen，17005-042）培养液包含两个组份：基础培养液1瓶（货号10724-011）+生长因子2管（货号37000-015 ）+1%GA(SD0038)。按照K-SFM培养液说明书，需额外添加Gentamicin Solution

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

PubMed=8977636; DOI=10.1002/(SICI)1097-0045(199612)29:6

Webber M.M., Bello D., Quader S.T.A.

Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines.

Prostate 29:386-394(1996)

PubMed=9214605; DOI=10.1093/carcin/18.6.1215

Bello D., Webber M.M., Kleinman H.K., Wartinger D.D., Rhim J.S.

Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18.

Carcinogenesis 18:1215-1223(1997)

PubMed=9214606; DOI=10.1093/carcin/18.6.1225

Webber M.M., Bello D., Kleinman H.K., Hoffman M.P.

Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells.

Carcinogenesis 18:1225-1231(1997)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

PubMed=22275356; DOI=10.1074/jbc.M111.302547

Lynch T.P., Ferrer C.M., Jackson S.R., Shahriari K.S., Vosseller K., Reginato M.J.

Critical role of O-linked beta-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis.

J. Biol. Chem. 287:11070-11081(2012)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30787054; DOI=10.1158/1055-9965.EPI-18-1132

Hooker S.E., Woods-Burnham L., Bathina M., Lloyd S.M., Gorjala P., Mitra R., Nonn L., Kimbro K.S., Kittles R.

Genetic ancestry analysis reveals misclassification of commonly used cancer cell lines.

Cancer Epidemiol. Biomarkers Prev. 28:1003-1009(2019)