C57BL/6小鼠卵巢成纤维细胞

AcceGen C57BL/6 小鼠原代卵巢成纤维细胞分离自无特定病原体实验小鼠的组织。将组织种到T25瓶让细胞成对数爬开，一般在AcceGen完全生长培养基里培养3-7天可以长满，随后进行传代扩增。细胞从培养瓶分离出来立即冷冻保存在冻存管中，每支冻存管的细胞浓度不低于 1x10^6/毫升，冷冻运送。

小鼠原代卵巢成纤维细胞经检测不含细菌、酵母菌、真菌及支原体。采用抗 FSP1/S100A4 抗体，通过免疫荧光染色法对细胞进行标志物表达检测。在 AcceGen 公司指定的细胞培养条件下，细胞可按 1:2 的比例传代扩增 3～5 代。不建议对细胞进行反复冻融。

适用于该细胞培养的标准生化实验包括细胞间相互作用检测、逆转录 - 聚合酶链反应（RT-PCR）、蛋白质印迹法（Western blotting）、免疫共沉淀、免疫荧光染色、流式细胞术，或制备用于特定研究用途的细胞衍生物。

C57BL/6 Mouse Primary Ovarian Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Ovarian Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

培养基配方：

成纤维细胞培养基FGCM(P6001）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：基础培养基+5%DMSO+20%FBS（SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

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