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小鼠AcceGen原代细胞从无致病性实验小鼠组织中分离。小鼠细胞在预涂明胶溶液的T25组织培养瓶中生长0.5小时，一般在AcceGen细胞培养基中扩增培养3-7天。每瓶每毫升至少含有1x10^6细胞，并冷冻运送。小鼠细胞对细菌、酵母菌、真菌和支原体呈阴性。不建议反复冷冻和解冻细胞。细胞培养的标准生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫荧光染色、流式细胞术或产生用于所需研究应用的细胞衍生物。
Mouse Primary Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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