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产品描述/Products Description
背景介绍/context：
Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. Cells have the capabilties to:
Form adherent monolayers – ideal for 96- and 384-well formats for high throughput and high content cell-based assays.
To differentiate into a wide variety of mesenchymal subtypes, including osteogenic and chondrogenic lineages (but not adipogenic)– ideal for drug discovery for a wide variety of biological targets and basic research in bone, cartilage, metabolic and immunological diseases.
Passage up to 10X.
Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only.
Specifications:
Cell Type: mesenchymal progenitor cells
Species: human
Source: derived from human embryonic cell line, H9 (WA09)
Morphology: fibroblastic
Quantity: >1x10^6 cells per vial (viable post-thaw)
Shipping method: dry ice
Storage: liquid nitrogen
Growth Properties: adherent
proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine
Phenotyping: Cells are >80% CD90, CD105, CD166 and CD73 positive and<5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination.
Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.
间充质干细胞祖细胞来源于人胚胎细胞系H9 (WA09)。细胞中CD90、CD105、CD166、CD73阳性占80%，Oct4阳性占5%。细胞具有以下能力:
形成贴壁单分子膜-理想的96孔和384孔格式的高通量和高含量的细胞为基础的分析。
分化为多种间充质亚型，包括成骨和软骨源性谱系(但不包括脂肪源性)，是发现多种生物靶点和骨、软骨、代谢和免疫疾病基础研究的理想药物。
传代可达到10个倍增。
注:请在订购时包括30美元的干冰费用。价格仅针对美国和加拿大。
规格:
细胞类型:间充质祖细胞
种类:人
来源:来自人类胚胎细胞系,H9 (WA09)
形态:成纤维细胞
数量:每瓶1x10^6细胞(解冻后活细胞)
运输方法:干冰
保存方法:液态氮增长
属性:贴壁生长
增殖培养基:补充10%胎牛血清、Pen/Strep和l -谷氨酰胺的αMEM培养基
表现型:细胞中CD90、CD105、CD166、CD73阳性占80%，Oct4阳性占5%。
DNA指纹图谱:结果与单细胞的存在一致，没有培养交叉污染的证据。
病原体检测:该批次来自H9 hESC细胞系，已对乙型肝炎、丙型肝炎、HIV-1、HIV-2、HTLV-I/II、HSV1、HSV2、EBV和CMV进行检测。H9细胞系已经过测试，显示为阴性(由GIVF实验室进行)。还测试间充质细胞的无菌性和支原体污染。
注意事项：此产品仅供科研使用
