16HBE14o-人支气管上皮细胞系

背景介绍/context：

16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport. When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein. Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.

16HBE14o-是从一名1岁男性心肺患者身上分离出来的人支气管上皮细胞系，具有复制起源缺陷SV40质粒(pSVori-)。该细胞系保留了正常分化支气管上皮细胞的特征，包括鹅卵石状的形态，细胞角蛋白的表达，形成紧密连接的能力，以及定向离子运输。当生长在一个空气/液体的界面，纤毛可以被检测。与大多数其他呼吸细胞系相比，16HBE14o-表达高水平的囊性纤维化跨膜电导调节(CFTR) mRNA和蛋白。在包括16HE14o上皮细胞在内的多种细胞中，CFTR的表达与camp依赖的Cl-电导相关。

动物种别/Organism  人

组织来源 Tissue and Cell Type 支气管上皮细胞

形态/Morphology 上皮细胞样，贴壁生长 

注意事项：此产品仅供科研使用