P388D1小鼠淋巴样瘤细胞

1975年从患有淋巴肿瘤的DBA/2小鼠腹水中建立了小鼠P-388D1单核/巨噬细胞细胞系(Koren et al. 1975)。P-388D1细胞为椭圆形、圆形、松散贴壁细胞，单层生长。在被脂多糖或富尔宝-12-肉豆蔻酸-13-醋酸酯刺激后，它们产生IL-1和LAF(淋巴细胞激活因子)。细胞吞噬酶和乳胶珠，并在抗体依赖的细胞介导的细胞毒性系统中活跃。
组织来源 Tissue and Cell Type：淋巴瘤
形态Morphology：淋巴母细胞样，悬浮生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

90%1640(MD0200)+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：1640完全培养基（货号：MD0201）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：此产品仅供科研使用

CLPUB00459

Dawe C.J., Potter M.

Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro.

Am. J. Pathol. 33:603-603(1957)

PubMed=1167564

Koren H.S., Handwerger B.S., Wunderlich J.R.

Identification of macrophage-like characteristics in a cultured murine tumor line.

J. Immunol. 114:894-897(1975)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x

Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.

Murine macrophage cell lines can be ordered in a linear differentiation sequence.

Differentiation 32:157-164(1986)

PubMed=10516761; DOI=10.1038/sj.leu.2401518

Coleman A.E., Forest S.T., McNeil N.E., Kovalchuk A.L., Ried T., Janz S.

Cytogenetic analysis of the bipotential murine pre-B cell lymphoma, P388, and its derivative macrophage-like tumor, P388D1, using SKY and CGH.

Leukemia 13:1592-1600(1999)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)