HCE-T [HCET] 人角膜上皮细胞

HCE-T细胞系是通过一位49岁女性的角膜组织进行SV40病毒转化建立的，表现出典型的上皮细胞形态，且能够产生64kD角蛋白。HCE-T细胞系的基因特征与角膜上皮细胞相关，表达与角膜上皮细胞功能相关的基因，如角蛋白基因，被广泛用作研究角膜上皮生物学各个方面的模型系统，包括细胞粘附、迁移、分化以及对环境刺激和治疗剂的反应等方向。疾病模型：干眼症、角膜感染（细菌/病毒）、创伤修复及角膜新生血管化研究。药物开发：评估眼用药物渗透性、毒性测试（如防腐剂苯扎氯铵）及纳米载体递送效果。材料测试：隐形眼镜、人工角膜材料的生物相容性分析。机制研究：炎症信号通路（NF-κB、MAPK）、氧化应激及凋亡机制。

动物种别：人

组织来源：角膜

形态：上皮细胞样，贴壁生长

培养基配方：CEpGM角膜上皮细胞培养基（货号：P4101）    

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：90%FBS（SD0200)+10%DMSO

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

PubMed=7534282
Kaoru Araki-Sasaki, Yuichi Ohashi, Tetsuo Sasabe, Kozaburo Hayashi, Hitoshi Watanabe, Yasuo Tano, Hiroshi Handa;
An SV40-immortalized human corneal epithelial cell line and its characterization.
Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... Michael Rudolf Stratton;
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26900324; PMCID=PMC4734150
Debra Akin, Jeremy R.B. Newman, Lauren M. McIntyre, Stephen P. Sugrue;
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.
Mol. Vis. 22:40-60(2016)

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018
Marina Juretic, Bisera Jurisic Dukovski, Iva Krtalic, Stephan Reichl, Biserka Cetina-Cizmek, Jelena Filipovic-Grcic, Jasmina Lovric, Ivan Pepic;
HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?
Eur. J. Pharm. Sci. 104:23-30(2017)

DOI=10.22028/D291-35665
Anna-Klara Rubelowski;
Characterization of the HCE-T cell line (RCB 2280) and its comparison with primary limbal epithelial cell culture and primary corneal epithelial cells.
Thesis MD (2018); Saarland University; Saarbrucken; Germany

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

PubMed=31927639; DOI=10.1007/s00417-019-04563-0
Anna-Klara Rubelowski, Lorenz Latta, Priya Katiyar, Tanja Stachon, Barbara Kasmann-Kellner, Berthold Seitz, Nora Szentmary;
HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.
Graefes Arch. Clin. Exp. Ophthalmol. 258:565-575(2020)