BS-C-1 非洲绿猴肾细胞

非洲绿猴肾细胞BS-C-1，又称BSC-1、BSC1、Biologics Standards-Cercopithecus-1等。该细胞建系于1961年，是从非洲绿猴肾脏中分离获得的一种自发永生化细胞系，表现为上皮样形态。该细胞通过免疫过氧化物酶染色显示角蛋白阳性，核型为2n=60。此外，BS-C-1细胞是SV40载体转染的理想宿主，对水疱性口炎病毒、1型人类脊髓灰质炎病毒、SV40病毒敏感，常用于病毒诊断、检测和研究。

动物种别：非洲绿猴

组织来源： 肾脏

形态：上皮细胞样，贴壁生长

培养基配方：MEM完全培养基（货号：MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：90%FBS（SD0200)+10%DMSO

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

PubMed=14473108; DOI=10.4049/jimmunol.88.6.796
Harry Martin Meyer Jr., Hope Elizabeth Hopps, Nancy G. Rogers, Barbara E. Brooks, Barbara C. Bernheim, William P. Jones, Ananda Nisalak, Robert D. Douglas;
Studies on simian virus 40.
J. Immunol. 88:796-806(1962)

PubMed=14071033; DOI=10.4049/jimmunol.91.3.416
Hope Elizabeth Hopps, Barbara C. Bernheim, Ananda Nisalak, Joe Hin Tjio, Joseph Edward Smadel;
Biologic characteristics of a continuous kidney cell line derived from the African green monkey.
J. Immunol. 91:416-424(1963)

PubMed=14215895; DOI=10.2105/ajph.54.9.1522; PMCID=PMC1255005
Nathalie J. Schmidt, Edwin Herman Lennette, Carol W. Shon, Juanita Dennis;
The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses.
Am. J. Public Health Nations Health 54:1522-1530(1964)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4339410; DOI=10.1002/ijc.2910090210
Horacio Guillermo Suarez, Marie-France Bourali, Rene Wicker, Simone Estrade, Roland Cassingena;
Properties of an SV40-transformed African green monkey kidney (BSC-1) cell line.
Int. J. Cancer 9:324-333(1972)

PubMed=4203458
John C. Petricciani, Roslyn Elizabeth Wallace, Donald W. McCoy;
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

PubMed=303774; DOI=10.1073/pnas.74.11.5046; PMCID=PMC432095
Robert William Holley, Rosemary Armour, Julia H. Baldwin, Kenneth D. Brown, Yun-Chi Yeh;
Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by serum factors.
Proc. Natl. Acad. Sci. U.S.A. 74:5046-5050(1977)

PubMed=272650; DOI=10.1073/pnas.75.1.339; PMCID=PMC411243
Robert William Holley, Rosemary Armour, Julia H. Baldwin;
Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by low molecular weight nutrients.
Proc. Natl. Acad. Sci. U.S.A. 75:339-341(1978)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3024506; DOI=10.1152/ajprenal.1986.251.6.F954
Thomas J. Jentsch, Harald Matthes, Svea K. Keller, Michael Wiederholt;
Electrical properties of sodium bicarbonate symport in kidney epithelial cells (BSC-1).
Am. J. Physiol. 251:F954-F968(1986)

PubMed=28046048; DOI=10.1371/journal.pone.0169391; PMCID=PMC5207746
Silvia Dotti, Tina Lombardo, Riccardo Villa, Andrea Cacciamali, Cinzia Zanotti, Nadia Andrea Andreani, Stefano Cinotti, Maura Ferrari;
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:e0169391.1-e0169391.13(2017)

PubMed=33458215; DOI=10.1080/23802359.2020.1827067; PMCID=PMC7781988
Nataliya Kozhukhar, Mikhail F. Alexeyev;
The mitochondrial genome sequence of the BS-C-1 cell line is at odds with the reported derivation from Cercopithecus aethiops.
Mitochondrial DNA B. Resour. 5:3492-3494(2020)