Neuro-2a小鼠脑神经瘤细胞

Neuro-2A是由R.J. Klebe和F.H. Ruddle经A品系白鼠的自发肿瘤建立。取材于A品系小鼠，这个肿瘤小鼠C1300购自Jackson Laboratory。该细胞产生大量微管蛋白，此蛋白在神经细胞中起应答轴浆流动的收缩系统起作用。检测小鼠痘病毒为阴性。经无支原体鉴定入库。

组织来源：脑 

形态：神经元和阿米巴样干细胞，贴壁生长

培养基配方：

90%MEM（MD0300）+10%胎牛血清(SD0200)+G/A (SD0038)

完全培养基：MEM完全培养基（MD0301）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

PubMed=11470893; DOI=10.1073/pnas.151242598

Enari M., Flechsig E., Weissmann C.

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Differentiation of mouse Neuro 2A cells into dopamine neurons.

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PubMed=25193168; DOI=10.1074/mcp.M113.037291

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Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Mol. Cell. Proteomics 13:3410-3420(2014)

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SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)