K7M2 wt小鼠骨肉瘤成骨细胞

抗原表达: CD31 阳性 [PubMed:1315100] 产物: 八因子 [PubMed: 11315100]; 整合唾液酸蛋白 (BSP) [PubMed11315100]; biglyan [PubMed: 11315100]; decorrin [PubMed:1315100]; villin 2 (ezrin) [PubMed: 11325848]. 骨唾液酸蛋白, biglyan, decorrin, 和 osteopontin 的表达显示其骨家族细胞特性。经无支原体鉴定入库。

组织来源：器官: 骨；疾病: 骨肉瘤；来源转移灶: 肺

形态：成骨细胞样，贴壁生长

培养基配方：

90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培养基（MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

PubMed=11315100; DOI=10.1023/A:1006767007547

Khanna C., Prehn J., Yeung C., Caylor J., Tsokos M., Helman L.

An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential.

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PubMed=17671136; DOI=10.1158/1078-0432.CCR-07-0313

Gordon N., Koshkina N.V., Jia S.-F., Khanna C., Mendoza A., Worth L.L., Kleinerman E.S.

Corruption of the Fas pathway delays the pulmonary clearance of murine osteosarcoma cells, enhances their metastatic potential, and reduces the effect of aerosol gemcitabine.

Clin. Cancer Res. 13:4503-4510(2007)

PubMed=26320182; DOI=10.18632/oncotarget.5177

Ren L., Mendoza A., Zhu J., Briggs J.W., Halsey C., Hong E.S., Burkett S.S., Morrow J., Lizardo M.M., Osborne T., Li S.Q., Luu H.H., Meltzer P.S., Khanna C.

Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.

Oncotarget 6:29469-29481(2015)