266-6小鼠胰腺腺泡癌细胞

266-6是Robert E.Hammer于1985年用一只成年小鼠腺泡胰腺建系。用弹性蛋白酶I/SV-40t抗原融合基因诱导肿瘤细胞。该受体表达乙酰胆碱，毒蕈碱。这些细胞保持部分分化表型，并表达可检测水平的消化酶mRNAs。细胞对氨甲胆碱和胆囊收缩素有反应，但对P物质、分泌素或血管活性肠肽（VIP）无反应。细胞携带弹性蛋白酶/新霉素转基因。

组织来源： 胰腺，胰腺腺泡癌

形态：上皮细胞样，贴壁生长

培养基配方：

90%DMEM（MD0100)+10%FBS(SD0200)+14mM HEPES(SD0009)+1%GA(SD0038)

完全培养基：DMEM完全培养基（MD0127）

气相：空气，95%；二氧化碳，5%。 温度：37℃。

冻存条件：基础培养基+5%DMSO+20%FBS(SD0200)

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

声明：产品仅科研使用。

Kruse F, et al. The cell-specific elastase I enhancer comprises two domains [published erratum appears in Mol. Cell. Biol. 8: 1862, 1988]. Mol. Cell. Biol. 8: 893-902, 1988. PubMed: 3352608

Swift GH, et al. Differential requirements for cell-specific elastase I enhancer domains in transfected cells and transgenic mice. Genes Dev. 3: 687-696, 1989. PubMed: 2744460

Ornitz DM, et al. Elastase I promoter directs expression of human growth hormone and SV40 T antigen genes to pancreatic acinar cells in transgenic mice. Cold Spring Harbor Symp. Quant. Biol. 50: 399-409, 1985. PubMed: 3006998

Rose SD, et al. A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut. Mol. Cell. Biol. 14: 2048-2057, 1994. PubMed: 8114736

Swift GH, et al. An element of the elastase I enhancer is an overlapping bipartite binding site activated by a heteromeric factor. J. Biol. Chem. 269: 12809-12815, 1994. PubMed: 8175694