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Rat Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of Sprague-Dawley Rats. Prior to shipping, cells are immediately cryo-preserved in vials. Each vial contains at least 9 x10^6 cells and is delivered frozen. Rat Peritoneal Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry. Rat Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
