Sp2/0-Ag14小鼠骨髓瘤细胞

不分泌免疫球蛋白，对20μg/ml 8-氮鸟嘌呤有抗性，对HAT敏感。 检测发现鼠痘病毒阴性。 在本库通过支原体检测。

动物种别/Organism 小鼠，NIH/Swiss 

组织来源/Tissue and Cell Type  骨髓瘤

形态/Morphology 淋巴细胞样，悬浮生长

培养基和添加剂/Complete Growth Medium and Culture Conditions：

 90%DMEM(MD0100)+10%胎牛血清(SD0200)+G/A (SD0038)

DMEM完全培养基（货号：MD0101）

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

冻存条件：基础培养基+5%DMSO+20%FBS　

完全冻存液：免程序降温细胞冻存液（低血清）(SD0001)

注意事项：本产品仅供科研使用。

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Kohler G.

Immunoglobulin chain loss in hybridoma lines.

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CLPUB00447

Mulivor R.A., Suchy S.F.

1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.

(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)