人肺泡上皮细胞（ HPAEpiC）

肺泡上皮细胞(Pulmonary alveolar epithelial cells, PAEpiC)由肺泡I型和II型上皮细胞组成，占肺内表面积的99%以上。I型细胞是大的鳞状细胞，其薄的胞浆延伸覆盖了95%的内表面积>。它们含有水通道蛋白，并表现出任何哺乳动物细胞类型中最高的渗透性水渗透性。II型细胞占表面积的2-5%，产生、分泌和循环肺表面活性剂。目前公认的假设是，II型细胞通过调节肺中活跃的Na+运输来维持肺液稳态，而I型细胞是“惰性”细胞，只提供屏障功能，而不具有活性功能。最近的研究表明，I型细胞在调节离子和液体运输方面也很重要。来自AcceGen研究实验室的HPAEpiC是从人类肺组织中分离出来的。HPAEpiC在原代培养时低温保存并冷冻运送。每个冻存管至少含有1×10^6的细胞，使用CK-18、19和vimentin免疫荧光法验证HPAEpiC。HPAEpiC细胞对HIV-1, HBV, HCV，支原体，细菌，酵母和真菌检测呈阴性。HPAEpiC需要在AcceGen研究实验室提供的条件下进行培养。然而，这种细胞不建议扩大或长期培养，因为细胞传代后立即分化为I型肺泡上皮细胞，而I型肺泡上皮细胞在培养过程中不增殖。

Pulmonary alveolar epithelial cells (PAEpiC) comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide solely a barrier function, rather than having active functions. Recent study indicate that Type I cells are also important in regulating ion and fluid transport.HPAEpiC from AcceGen Research Laboratories are isolated from human lung tissue. HPAEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPAEpiC are characterized by immunofluorescent method with antibodies CK-18, -19, and vimentin. HPAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEpiC are guaranteed to further culture at the conditions provided by AcceGen Research Laboratories. However, this cell type is not recommend for expanding or long term cultures since the cells would differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.

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