人肝星状细胞-GFP

HLiStCs是由肝动脉灌注消化胶原酶引起的。将肝窦状MV内皮细胞与Kupffer细胞的混合物在消化液中分离。GFP-HLiStCs是在感染表达GFP的慢病毒颗粒后，从耐嘌呤霉素的HLiStCs中筛选出来的。细胞在P3代冻存提供。建议使用星状生长培养基进行细胞培养，当按照推荐描述的详细方案培养时，细胞可达8倍增。

HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the mixture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. GFP-HLiStCs are selected from Puromycin resistant HLiStCs after infected with GFP expressing lentiviral particles. The cells are shipped in frozen vials (the cells are provided @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity > 8 when cultured following the detailed protocol described below.

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