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  • 人皮肤成纤维细胞-GFP/LUC双标(新生儿)GFP-Luc-HNDFCs-Neo

人皮肤成纤维细胞-GFP/LUC双标(新生儿)GFP-Luc-HNDFCs-Neo

货号:ABC-357

价格: ¥询价

规格:
1 vial
数量: - +
  • 人皮肤成纤维细胞-GFP/LUC双标(新生儿)GFP-Luc-HNDFCs-Neo
产品描述/Products Description

从正常新生儿包皮组织中分离出HNDFCs-Neo细胞,并在P1代转染表达GFP和荧光素酶的慢病毒颗粒。选择耐嘌呤霉素的GFP-Luc-HNDFCs-Neo细胞,在P3代进行冻存运输。建议使用成纤维细胞生长培养基进行细胞培养,当按照推荐详细方案进行培养时,细胞可达14倍增。

HNDFCs-Neo cells are isolated from normal neonatal foreskin tissue samples and transfected with GFP and Luciferase expressing Lentiviral particles at passage one. Puromycin resistant GFP-Luc-HNDFCs-Neo are selected and shipped in frozen vial (the cell are provided @passage 3). Fibroblast Growth medium is recommended for cell culture and these cells have an average minimum population doubling levels > 14 when cultured following the detailed protocol described below.


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