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糖尿病自发性突变(Lepr/db)表现为病理性肥胖、慢性高血糖、胰腺细胞萎缩和降血脂症，从小鼠动脉中分离出BKS db小鼠动脉内皮细胞。小鼠动脉内皮细胞在涂有明胶溶液的T25组织培养瓶中生长2分钟，一般在AcceGen完全生长培养基中扩增培养3-7天。发货的细胞为第三代的细胞，每瓶每毫升至少含有0.5×106个细胞。我们分离原代内皮细胞的方法是在已有方法和我们的专利方法的基础上发展起来的。这些细胞被预涂PECAM-1抗体，随后应用预涂二抗的磁珠分选。
BKS db Mouse Artery Endothelial Cells are isolated from the artery of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
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