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小鼠腹腔巨噬细胞来源于无病原体实验室成年小鼠的腹腔。每瓶含有至少8个x10^6细胞，冷冻运送。小鼠腹腔巨噬细胞对细菌、酵母、真菌和支原体呈阴性。细胞可在AcceGen指定的细胞培养条件下在多孔培养板上进行扩增，以备实验使用。不建议反复冷冻和解冻细胞。通过流式细胞术检测小鼠腹腔巨噬细胞抗体CD11b标记物的表达。细胞培养的标准生化程序包括RT-PCR、Western blotting、免疫沉淀、免疫荧光染色、流式细胞术或产生用于所需研究应用的细胞衍生物。
Mouse Resident Peritoneal Macrophages are derived from the peritoneal cavity of pathogen-free laboratory adult mice. Each vial contains at least 8 x10^6 cells and is delivered frozen. Mouse Resident Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Mouse Resident Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
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