BALB/c小鼠卵巢成纤维细胞

从无致病性实验小鼠组织中分离得到BALB/c小鼠AcceGen原代卵巢成纤维细胞。小鼠原代卵巢成纤维细胞在预涂明胶溶液的T25组织培养瓶中生长0.5小时，一般在AcceGen培养完全生长培养基中培养3-7天。然后文化被扩展。装运前，细胞从瓶中分离出来，立即冷冻保存在小瓶中。每瓶至少含有1x10^6细胞每毫升，并冷冻运送。小鼠原代卵巢成纤维细胞对细菌、酵母、真菌和支原体呈阴性。免疫荧光染色检测细胞标记物的表达，抗体为anti-FSP1/S100A4。在AcceGen规定的细胞培养条件下，细胞可按1:2的传代比例扩增3-5代。不建议反复冷冻和解冻细胞。细胞培养的标准生化程序包括测定细胞间相互作用、RT-PCR、Western blotting、免疫沉淀、免疫荧光染色、流式细胞术或产生用于所需研究应用的细胞衍生物。

BALB/c Mouse Primary Ovarian Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Ovarian Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

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